Separation of endo-polygalacturonase using aqueous two-phase partitioning
نویسندگان
چکیده
منابع مشابه
Separation of endo-polygalacturonase using aqueous two-phase partitioning.
The partitioning of endo-polygalacturonase (endo-PG) in polyethylene glycol (PEG)-polyvinyl alcohol (PVA10000) and PEG-hydroxypropyl starch (Reppal PES100) aqueous two-phase systems was studied, and revealed the possibility of using aqueous two-phase extraction to purify and concentrate endo-PG from its clarified fermentation broth. For the PEG8000-PVA10000 system, endo-PG presented in the ferm...
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Aqueous two-phase partitioning of endo-polygalacturonase (endo-PG) produced by Kluyveromyces marxianus strains was studied, on systems containing the thermoseparating polymer Ucon 50-HB-5100 (a random copolymer of 50% ethylene oxide (EO) and 50% propylene oxide (PO)) as one of the phase-forming compounds. Ucon 50-HB-5100 (Ucon)–polyvinyl alcohol (PVA 10,000), Ucon 50-HB-5100–hydroxypropyl starc...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2001
ISSN: 0021-9673
DOI: 10.1016/s0021-9673(01)01161-x